PdMYB118, isolated from a red leaf mutant of Populus deltoids, is a new transcription factor regulating anthocyanin biosynthesis in poplar.
Identifieur interne : 000799 ( Main/Exploration ); précédent : 000798; suivant : 000800PdMYB118, isolated from a red leaf mutant of Populus deltoids, is a new transcription factor regulating anthocyanin biosynthesis in poplar.
Auteurs : Haihai Wang [République populaire de Chine] ; Xiaoqing Wang [République populaire de Chine] ; Weimeng Song [République populaire de Chine] ; Yan Bao [République populaire de Chine] ; Yanli Jin [République populaire de Chine] ; Chunmei Jiang [République populaire de Chine] ; Cuiting Wang [République populaire de Chine] ; Bei Li [République populaire de Chine] ; Hongxia Zhang [République populaire de Chine]Source :
- Plant cell reports [ 1432-203X ] ; 2019.
Descripteurs français
- KwdFr :
- Anthocyanes (métabolisme), Facteurs de transcription (génétique), Facteurs de transcription (métabolisme), Feuilles de plante (génétique), Feuilles de plante (métabolisme), Mutation (génétique), Populus (génétique), Populus (métabolisme), Protéines végétales (génétique), Protéines végétales (métabolisme), Régulation de l'expression des gènes végétaux (génétique), Régulation de l'expression des gènes végétaux (physiologie), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (métabolisme).
- MESH :
- génétique : Facteurs de transcription, Feuilles de plante, Mutation, Populus, Protéines végétales, Régulation de l'expression des gènes végétaux, Végétaux génétiquement modifiés.
- métabolisme : Anthocyanes, Facteurs de transcription, Feuilles de plante, Populus, Protéines végétales, Végétaux génétiquement modifiés.
- physiologie : Régulation de l'expression des gènes végétaux.
English descriptors
- KwdEn :
- Anthocyanins (metabolism), Gene Expression Regulation, Plant (genetics), Gene Expression Regulation, Plant (physiology), Mutation (genetics), Plant Leaves (genetics), Plant Leaves (metabolism), Plant Proteins (genetics), Plant Proteins (metabolism), Plants, Genetically Modified (genetics), Plants, Genetically Modified (metabolism), Populus (genetics), Populus (metabolism), Transcription Factors (genetics), Transcription Factors (metabolism).
- MESH :
- chemical , genetics : Plant Proteins, Transcription Factors.
- chemical , metabolism : Anthocyanins, Plant Proteins, Transcription Factors.
- genetics : Gene Expression Regulation, Plant, Mutation, Plant Leaves, Plants, Genetically Modified, Populus.
- metabolism : Plant Leaves, Plants, Genetically Modified, Populus.
- physiology : Gene Expression Regulation, Plant.
Abstract
KEY MESSAGE
A new anthocyanin biosynthesis transcription factor PdMYB118, which could be used for the genetic engineering of colorful tree species, was indentified from a red leaf mutant of Populus deltoids. In higher plants, the biosynthesis of anthocyanins is regulated by several classes of transcription factors (TFs), including R2R3-MYB, bHLH and WD-repeat proteins. In this work, we isolated an MYB gene regulating anthocyanin biosynthesis from a red leaf mutant of Populus deltoids, which accumulated more anthocyanins in the leaves and showed higher expression levels of anthocyanin biosynthesis genes than did the wild type. Gene expression analyses of all TFs regulating anthocyanin biosynthesis demonstrated that only a MYB118 homologous gene, PdMYB118, was up-regulated in the mutant compared with the wide type. Subcellular localization analyses in poplar leaf mesophyll protoplasts showed that PdMYB118-YFP fusion protein was specifically located in nucleus. When transiently expressed in poplar leaf protoplasts, PdMYB118 specifically promoted the expression of anthocyanidin biosynthesis genes. Dual-luciferase assays revealed that PdMYB118 can directly activate the promoters of these genes. When overexpressed in Shanxin Yang (P. davidiana × P. bolleana), a hybrid clone commercially grown for landscaping in the northern part of China, transgenic plants overexpressing PdMYB118 produced more anthocyanins in the leaves and turned their color into redness when grown in both greenhouse and field. Consistently, transcripts of some important anthocyanidin biosynthesis genes were significantly increased in the leaves of transgenic plants. All these results indicate that PdMYB118 functions as an essential transcription factor regulating anthocyanin biosynthesis in poplar and could be used for the genetic engineering of colorful tree species.
DOI: 10.1007/s00299-019-02413-1
PubMed: 31147728
Affiliations:
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Le document en format XML
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Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation></author><author><name sortKey="Li, Bei" sort="Li, Bei" uniqKey="Li B" first="Bei" last="Li">Bei Li</name><affiliation wicri:level="1"><nlm:affiliation>College of Agriculture, Ludong University, 186 Hongqizhong Road, Yantai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Agriculture, Ludong University, 186 Hongqizhong Road, Yantai</wicri:regionArea><wicri:noRegion>Yantai</wicri:noRegion></affiliation><affiliation 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Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>College of Agriculture, Ludong University, 186 Hongqizhong Road, Yantai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Agriculture, Ludong University, 186 Hongqizhong Road, Yantai</wicri:regionArea><wicri:noRegion>Yantai</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Xiaoqing" sort="Wang, Xiaoqing" uniqKey="Wang X" first="Xiaoqing" last="Wang">Xiaoqing Wang</name><affiliation wicri:level="1"><nlm:affiliation>Forestry and Pomology Research Institute, Shanghai Academy of Agriculture Sciences, 1000 Jinqi Road, Shanghai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Forestry and Pomology Research Institute, Shanghai Academy of Agriculture Sciences, 1000 Jinqi Road, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation></author><author><name sortKey="Song, Weimeng" sort="Song, Weimeng" uniqKey="Song W" first="Weimeng" last="Song">Weimeng Song</name><affiliation wicri:level="1"><nlm:affiliation>National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>College of Agriculture, Ludong University, 186 Hongqizhong Road, Yantai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Agriculture, Ludong University, 186 Hongqizhong Road, Yantai</wicri:regionArea><wicri:noRegion>Yantai</wicri:noRegion></affiliation></author><author><name sortKey="Bao, Yan" sort="Bao, Yan" uniqKey="Bao Y" first="Yan" last="Bao">Yan Bao</name><affiliation wicri:level="1"><nlm:affiliation>College of Agriculture, Ludong University, 186 Hongqizhong Road, Yantai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Agriculture, Ludong University, 186 Hongqizhong Road, Yantai</wicri:regionArea><wicri:noRegion>Yantai</wicri:noRegion></affiliation></author><author><name sortKey="Jin, Yanli" sort="Jin, Yanli" uniqKey="Jin Y" first="Yanli" last="Jin">Yanli Jin</name><affiliation wicri:level="1"><nlm:affiliation>National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation></author><author><name sortKey="Jiang, Chunmei" sort="Jiang, Chunmei" uniqKey="Jiang C" first="Chunmei" last="Jiang">Chunmei Jiang</name><affiliation wicri:level="3"><nlm:affiliation>Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, 368 Youyi Avenue, Wuhan, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, 368 Youyi Avenue, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Wang, Cuiting" sort="Wang, Cuiting" uniqKey="Wang C" first="Cuiting" last="Wang">Cuiting Wang</name><affiliation wicri:level="1"><nlm:affiliation>National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation></author><author><name sortKey="Li, Bei" sort="Li, Bei" uniqKey="Li B" first="Bei" last="Li">Bei Li</name><affiliation wicri:level="1"><nlm:affiliation>College of Agriculture, Ludong University, 186 Hongqizhong Road, Yantai, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Agriculture, Ludong University, 186 Hongqizhong Road, Yantai</wicri:regionArea><wicri:noRegion>Yantai</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Institute for Advanced Study of Coastal Ecology, and the Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Ludong University, 186 Hongqizhong Road, Yantai, 264025, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for Advanced Study of Coastal Ecology, and the Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Ludong University, 186 Hongqizhong Road, Yantai, 264025</wicri:regionArea><wicri:noRegion>264025</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Hongxia" sort="Zhang, Hongxia" uniqKey="Zhang H" first="Hongxia" last="Zhang">Hongxia Zhang</name><affiliation wicri:level="1"><nlm:affiliation>College of Agriculture, Ludong University, 186 Hongqizhong Road, Yantai, China. hxzhang@sibs.ac.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Agriculture, Ludong University, 186 Hongqizhong Road, Yantai</wicri:regionArea><wicri:noRegion>Yantai</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Institute for Advanced Study of Coastal Ecology, and the Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Ludong University, 186 Hongqizhong Road, Yantai, 264025, China. hxzhang@sibs.ac.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute for Advanced Study of Coastal Ecology, and the Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Ludong University, 186 Hongqizhong Road, Yantai, 264025</wicri:regionArea><wicri:noRegion>264025</wicri:noRegion></affiliation></author></analytic><series><title level="j">Plant cell reports</title><idno type="eISSN">1432-203X</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anthocyanins (metabolism)</term><term>Gene Expression Regulation, Plant (genetics)</term><term>Gene Expression Regulation, Plant (physiology)</term><term>Mutation (genetics)</term><term>Plant Leaves (genetics)</term><term>Plant Leaves (metabolism)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (metabolism)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>Transcription Factors (genetics)</term><term>Transcription Factors (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anthocyanes (métabolisme)</term><term>Facteurs de transcription (génétique)</term><term>Facteurs de transcription (métabolisme)</term><term>Feuilles de plante (génétique)</term><term>Feuilles de plante (métabolisme)</term><term>Mutation (génétique)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Régulation de l'expression des gènes végétaux (génétique)</term><term>Régulation de l'expression des gènes végétaux (physiologie)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Végétaux génétiquement modifiés (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Anthocyanins</term><term>Plant Proteins</term><term>Transcription Factors</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Mutation</term><term>Plant Leaves</term><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Facteurs de transcription</term><term>Feuilles de plante</term><term>Mutation</term><term>Populus</term><term>Protéines végétales</term><term>Régulation de l'expression des gènes végétaux</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plant Leaves</term><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Anthocyanes</term><term>Facteurs de transcription</term><term>Feuilles de plante</term><term>Populus</term><term>Protéines végétales</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Régulation de l'expression des gènes végétaux</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Gene Expression Regulation, Plant</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>KEY MESSAGE</b></p><p>A new anthocyanin biosynthesis transcription factor PdMYB118, which could be used for the genetic engineering of colorful tree species, was indentified from a red leaf mutant of Populus deltoids. In higher plants, the biosynthesis of anthocyanins is regulated by several classes of transcription factors (TFs), including R2R3-MYB, bHLH and WD-repeat proteins. In this work, we isolated an MYB gene regulating anthocyanin biosynthesis from a red leaf mutant of Populus deltoids, which accumulated more anthocyanins in the leaves and showed higher expression levels of anthocyanin biosynthesis genes than did the wild type. Gene expression analyses of all TFs regulating anthocyanin biosynthesis demonstrated that only a MYB118 homologous gene, PdMYB118, was up-regulated in the mutant compared with the wide type. Subcellular localization analyses in poplar leaf mesophyll protoplasts showed that PdMYB118-YFP fusion protein was specifically located in nucleus. When transiently expressed in poplar leaf protoplasts, PdMYB118 specifically promoted the expression of anthocyanidin biosynthesis genes. Dual-luciferase assays revealed that PdMYB118 can directly activate the promoters of these genes. When overexpressed in Shanxin Yang (P. davidiana × P. bolleana), a hybrid clone commercially grown for landscaping in the northern part of China, transgenic plants overexpressing PdMYB118 produced more anthocyanins in the leaves and turned their color into redness when grown in both greenhouse and field. Consistently, transcripts of some important anthocyanidin biosynthesis genes were significantly increased in the leaves of transgenic plants. All these results indicate that PdMYB118 functions as an essential transcription factor regulating anthocyanin biosynthesis in poplar and could be used for the genetic engineering of colorful tree species.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31147728</PMID><DateCompleted><Year>2019</Year><Month>08</Month><Day>06</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-203X</ISSN><JournalIssue CitedMedium="Internet"><Volume>38</Volume><Issue>8</Issue><PubDate><Year>2019</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Plant cell reports</Title><ISOAbbreviation>Plant Cell Rep</ISOAbbreviation></Journal><ArticleTitle>PdMYB118, isolated from a red leaf mutant of Populus deltoids, is a new transcription factor regulating anthocyanin biosynthesis in poplar.</ArticleTitle><Pagination><MedlinePgn>927-936</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00299-019-02413-1</ELocationID><Abstract><AbstractText Label="KEY MESSAGE" NlmCategory="UNASSIGNED">A new anthocyanin biosynthesis transcription factor PdMYB118, which could be used for the genetic engineering of colorful tree species, was indentified from a red leaf mutant of Populus deltoids. In higher plants, the biosynthesis of anthocyanins is regulated by several classes of transcription factors (TFs), including R2R3-MYB, bHLH and WD-repeat proteins. In this work, we isolated an MYB gene regulating anthocyanin biosynthesis from a red leaf mutant of Populus deltoids, which accumulated more anthocyanins in the leaves and showed higher expression levels of anthocyanin biosynthesis genes than did the wild type. Gene expression analyses of all TFs regulating anthocyanin biosynthesis demonstrated that only a MYB118 homologous gene, PdMYB118, was up-regulated in the mutant compared with the wide type. Subcellular localization analyses in poplar leaf mesophyll protoplasts showed that PdMYB118-YFP fusion protein was specifically located in nucleus. When transiently expressed in poplar leaf protoplasts, PdMYB118 specifically promoted the expression of anthocyanidin biosynthesis genes. Dual-luciferase assays revealed that PdMYB118 can directly activate the promoters of these genes. When overexpressed in Shanxin Yang (P. davidiana × P. bolleana), a hybrid clone commercially grown for landscaping in the northern part of China, transgenic plants overexpressing PdMYB118 produced more anthocyanins in the leaves and turned their color into redness when grown in both greenhouse and field. Consistently, transcripts of some important anthocyanidin biosynthesis genes were significantly increased in the leaves of transgenic plants. All these results indicate that PdMYB118 functions as an essential transcription factor regulating anthocyanin biosynthesis in poplar and could be used for the genetic engineering of colorful tree species.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Haihai</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Agriculture, Ludong University, 186 Hongqizhong Road, Yantai, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Xiaoqing</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Forestry and Pomology Research Institute, Shanghai Academy of Agriculture Sciences, 1000 Jinqi Road, Shanghai, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Song</LastName><ForeName>Weimeng</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Agriculture, Ludong University, 186 Hongqizhong Road, Yantai, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bao</LastName><ForeName>Yan</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>College of Agriculture, Ludong University, 186 Hongqizhong Road, Yantai, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jin</LastName><ForeName>Yanli</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Chunmei</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Sciences, Hubei University, 368 Youyi Avenue, Wuhan, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Cuiting</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Bei</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>College of Agriculture, Ludong University, 186 Hongqizhong Road, Yantai, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Institute for Advanced Study of Coastal Ecology, and the Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Ludong University, 186 Hongqizhong Road, Yantai, 264025, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Hongxia</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>College of Agriculture, Ludong University, 186 Hongqizhong Road, Yantai, China. hxzhang@sibs.ac.cn.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Institute for Advanced Study of Coastal Ecology, and the Key Laboratory of Molecular Module-Based Breeding of High Yield and Abiotic Resistant Plants in the Universities of Shandong, Ludong University, 186 Hongqizhong Road, Yantai, 264025, China. hxzhang@sibs.ac.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>2018ZX08020002-003-004</GrantID><Agency>the National Key Program on Transgenic Research</Agency><Country></Country></Grant><Grant><GrantID>LYCX03-2018-18</GrantID><Agency>the Forestry Science and Technology Innovation Project of Shandong province of China</Agency><Country></Country></Grant><Grant><GrantID>2016YFD0600106</GrantID><Agency>the National Key R & D Program of China</Agency><Country></Country></Grant><Grant><GrantID>2016ZX08004-002-006</GrantID><Agency>the National Major Project of GMO New Species Cultivation</Agency><Country></Country></Grant><Grant><GrantID>SDAIT-02-05</GrantID><Agency>The Modern Agricultural Industry Technology System Innovation Team of Shandong Province of China</Agency><Country></Country></Grant><Grant><GrantID>DD20160025</GrantID><Agency>Comprehensive Surveys on Saline Lake Lithium and other New Energy Resources in the North Tibetan Plateau</Agency><Country></Country></Grant><Grant><GrantID>31870576</GrantID><Agency>National Natural Science Foundation of China (CN)</Agency><Country></Country></Grant><Grant><GrantID>31871625</GrantID><Agency>National Natural Science Foundation of China</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>05</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Plant Cell Rep</MedlineTA><NlmUniqueID>9880970</NlmUniqueID><ISSNLinking>0721-7714</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000872">Anthocyanins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014157">Transcription Factors</NameOfSubstance></Chemical></ChemicalList><MeshHeadingList><MeshHeading><DescriptorName UI="D000872" MajorTopicYN="N">Anthocyanins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009154" MajorTopicYN="N">Mutation</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014157" MajorTopicYN="N">Transcription Factors</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Anthocyanins</Keyword><Keyword MajorTopicYN="N">PdMYB118</Keyword><Keyword MajorTopicYN="N">Poplar</Keyword><Keyword MajorTopicYN="N">Red leaf</Keyword><Keyword MajorTopicYN="N">Transcription factor</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>03</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>04</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>5</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>8</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>6</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31147728</ArticleId><ArticleId IdType="doi">10.1007/s00299-019-02413-1</ArticleId><ArticleId IdType="pii">10.1007/s00299-019-02413-1</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2000 Dec 5;97(25):13579-84</Citation><ArticleIdList><ArticleId IdType="pubmed">11095727</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2000 Dec;12(12):2383-2394</Citation><ArticleIdList><ArticleId IdType="pubmed">11148285</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2001 Jun;126(2):485-93</Citation><ArticleIdList><ArticleId IdType="pubmed">11402179</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2001 Sep;13(9):2099-114</Citation><ArticleIdList><ArticleId IdType="pubmed">11549766</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2001 Dec;127(4):1466-75</Citation><ArticleIdList><ArticleId IdType="pubmed">11743090</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2003 Dec;133(4):1831-42</Citation><ArticleIdList><ArticleId IdType="pubmed">14605235</ArticleId></ArticleIdList></Reference><Reference><Citation>J Agric Food Chem. 2005 Jan 26;53(2):272-81</Citation><ArticleIdList><ArticleId IdType="pubmed">15656661</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Methods. 2005 Dec 18;1:13</Citation><ArticleIdList><ArticleId IdType="pubmed">16359558</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2006 Feb;18(2):283-94</Citation><ArticleIdList><ArticleId IdType="pubmed">16399804</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2006;172(1):47-62</Citation><ArticleIdList><ArticleId IdType="pubmed">16945088</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2007 Feb;49(3):414-27</Citation><ArticleIdList><ArticleId IdType="pubmed">17181777</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2007 Mar;143(3):1347-61</Citation><ArticleIdList><ArticleId IdType="pubmed">17208963</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Protoc. 2007;2(7):1565-72</Citation><ArticleIdList><ArticleId IdType="pubmed">17585298</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Physiol. 2008 Dec;49(12):1818-29</Citation><ArticleIdList><ArticleId IdType="pubmed">18974195</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2009 Feb;149(2):981-93</Citation><ArticleIdList><ArticleId IdType="pubmed">19091872</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2009 Jun;150(2):924-41</Citation><ArticleIdList><ArticleId IdType="pubmed">19395405</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 2010 Apr;72(6):607-20</Citation><ArticleIdList><ArticleId IdType="pubmed">20112051</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 2010 Nov;74(4-5):367-80</Citation><ArticleIdList><ArticleId IdType="pubmed">20803312</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 2011 May;62(8):2465-83</Citation><ArticleIdList><ArticleId IdType="pubmed">21278228</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 2011 Nov;30(11):2037-44</Citation><ArticleIdList><ArticleId IdType="pubmed">21717184</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Sci. 2011 Sep;181(3):219-29</Citation><ArticleIdList><ArticleId IdType="pubmed">21763532</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2012 Jul;159(3):1204-20</Citation><ArticleIdList><ArticleId IdType="pubmed">22566493</ArticleId></ArticleIdList></Reference><Reference><Citation>J Exp Bot. 2013 Jul;64(10):2847-57</Citation><ArticleIdList><ArticleId IdType="pubmed">23667043</ArticleId></ArticleIdList></Reference><Reference><Citation>Tree Physiol. 2013 Aug;33(8):878-86</Citation><ArticleIdList><ArticleId IdType="pubmed">23939552</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2014 Feb;77(3):367-79</Citation><ArticleIdList><ArticleId IdType="pubmed">24274116</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2014 Apr;202(1):174-87</Citation><ArticleIdList><ArticleId IdType="pubmed">24350984</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Signal Behav. 2014;9(1):e27522</Citation><ArticleIdList><ArticleId IdType="pubmed">24393776</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Plant Sci. 2015 Mar;20(3):176-85</Citation><ArticleIdList><ArticleId IdType="pubmed">25577424</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2015 Mar;167(3):693-710</Citation><ArticleIdList><ArticleId IdType="pubmed">25624398</ArticleId></ArticleIdList></Reference><Reference><Citation>Tree Physiol. 2015 Jun;35(6):663-77</Citation><ArticleIdList><ArticleId IdType="pubmed">25877769</ArticleId></ArticleIdList></Reference><Reference><Citation>Tree Physiol. 2016 Sep;36(9):1162-76</Citation><ArticleIdList><ArticleId IdType="pubmed">27259636</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Biotechnol J. 2017 Oct;15(10):1309-1321</Citation><ArticleIdList><ArticleId IdType="pubmed">28258966</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2017 May;174(1):154-171</Citation><ArticleIdList><ArticleId IdType="pubmed">28348066</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell Rep. 2017 Aug;36(8):1263-1276</Citation><ArticleIdList><ArticleId IdType="pubmed">28523445</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Mol Biol. 2018 Sep;98(1-2):1-18</Citation><ArticleIdList><ArticleId IdType="pubmed">30167900</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Hubei</li></region><settlement><li>Wuhan</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Haihai" sort="Wang, Haihai" uniqKey="Wang H" first="Haihai" last="Wang">Haihai Wang</name></noRegion><name sortKey="Bao, Yan" sort="Bao, Yan" uniqKey="Bao Y" first="Yan" last="Bao">Yan Bao</name><name sortKey="Jiang, Chunmei" sort="Jiang, Chunmei" uniqKey="Jiang C" first="Chunmei" last="Jiang">Chunmei Jiang</name><name sortKey="Jin, Yanli" sort="Jin, Yanli" uniqKey="Jin Y" first="Yanli" last="Jin">Yanli Jin</name><name sortKey="Li, Bei" sort="Li, Bei" uniqKey="Li B" first="Bei" last="Li">Bei Li</name><name sortKey="Li, Bei" sort="Li, Bei" uniqKey="Li B" first="Bei" last="Li">Bei Li</name><name sortKey="Song, Weimeng" sort="Song, Weimeng" uniqKey="Song W" first="Weimeng" last="Song">Weimeng Song</name><name sortKey="Song, Weimeng" sort="Song, Weimeng" uniqKey="Song W" first="Weimeng" last="Song">Weimeng Song</name><name sortKey="Wang, Cuiting" sort="Wang, Cuiting" uniqKey="Wang C" first="Cuiting" last="Wang">Cuiting Wang</name><name sortKey="Wang, Haihai" sort="Wang, Haihai" uniqKey="Wang H" first="Haihai" last="Wang">Haihai Wang</name><name sortKey="Wang, Xiaoqing" sort="Wang, Xiaoqing" uniqKey="Wang X" first="Xiaoqing" last="Wang">Xiaoqing Wang</name><name sortKey="Zhang, Hongxia" sort="Zhang, Hongxia" uniqKey="Zhang H" first="Hongxia" last="Zhang">Hongxia Zhang</name><name sortKey="Zhang, Hongxia" sort="Zhang, Hongxia" uniqKey="Zhang H" first="Hongxia" last="Zhang">Hongxia Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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